Regulatory

Part:BBa_K2148016:Design

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-13)


C.reinhardtii chloroplast rbcL 3UTR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 490
    Illegal XbaI site found at 478
    Illegal XbaI site found at 520
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 490
    Illegal NheI site found at 42
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 490
    Illegal BamHI site found at 508
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 490
    Illegal XbaI site found at 478
    Illegal XbaI site found at 520
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 490
    Illegal XbaI site found at 478
    Illegal XbaI site found at 520
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Most literature of usage was from work done by Dr Saul Purton (UCL)


Source

Chlamydomonas reinhardtii

References